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KMID : 0903519850280030209
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1985 Volume.28 No. 3 p.209 ~ p.217
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Studies on the Extracellular Protein Production by Bacillus sp .
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Abstract
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Seventeen extracellular protein producing bacteria were isolated from soil samples, among which T219 strain having a strong capability of producing the protein was selected and identified for investigation of biological characteristics. The factors which affect the protein production were investigated and the results are summarized as follows.
T219 strain which produces the most extracellular protein was identified as Bacillus sp. Optimum temperature and pH for production of the extracellular protein by T219 strain were 25℃ and 7.5 respectively. Almost no activities of protease and amylase were observed in the protein produced by the protein producing bacteria. In the medium containing yeast extract, the cell growth was moderately high, but almost no accumulation of protein was observed. However, polypeptone had significant effects on both the cell growth and the protein accumulation. The addition of glycine and L-isoleucine to the medium containing polypeptone, yeast extract and meat extract had a great effect on the protein production; 4㎎/㎖ of protein accumulation was observed.
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